Journal: Scientific Reports

Article Title: Revisiting bioluminescence and sucrose utilization in aquatic pathogens Vibrio harveyi and V. campbellii using genome-wide in silico mapping and phenotyping

doi: 10.1038/s41598-026-37651-3

Figure Lengend Snippet: Maximum likelihood Phylogenetic analysis of concatenated sequence of luminescence (LuxCDABEG) ( A ) and ScrRAKB ( B ) in V. harveyi and V. campbellii. The strains having full set of LuxCDABEG and ScrRAKB were selected for phyologenetic analysis. V. vulnificus ATCC 43382 served as outgroup for bioluminescence and V. alginolyticus ATCC 17749 for ScrRAKB clustering. Strains sequenced in the present study has been shown in blue color. Bootstrap value of more than 0.75 has been shown as star. Increasing size of star indicates higher bootstrap value. The tree was visualized and annotated using iTOL. Vh, V. harveyi ; Vc, V. campbellii ; Vv, V. vulnificus; Va, V. alginolyticus

Article Snippet: 1 , ScrR , AGV19641.1 , Sucrose operon repressor , , , V. alginolyticus ATCC 17749 , , .

Techniques: Sequencing

Journal: Journal of Ovarian Research

Article Title: Hypoxia-inducible factor-1α induces CX3CR1 expression and promotes the epithelial to mesenchymal transition (EMT) in ovarian cancer cells

doi: 10.1186/s13048-019-0517-1

Figure Lengend Snippet: CX3CR1 and HIF-1α expression by OvCa tissues: Ovarian tissues from normal and various cancer stages [well-differentiated (Stage I), moderately differentiated (Stage II), and poorly differentiated (Stage III)] were stained with anti-CX3CR1 and anti-HIF-1α antibodies. Magenta (AP) color shows CX3CR1, and Brown (DAB) color shows HIF-1α staining. An Aperio ScanScope CS system with a 40X objective captured digital images of each tissue. Representative cases are immuno-intensities of CX3CR1 and HIF-1α using image analysis Aperio ImageScope v.6.25 software

Article Snippet: After washing with PBS-T, tissue sections were incubated with streptavidin-horseradish peroxidase (HRP, Biolegend) (HIF-1α) or streptavidin-alkaline phosphatase (Jackson ImmunoResearch) (CX3CR1) and developed in diaminobenzidine and/or alkaline phosphatase red chromogen coloring agent.

Techniques: Expressing, Staining, Software

Journal: Journal of Ovarian Research

Article Title: Hypoxia-inducible factor-1α induces CX3CR1 expression and promotes the epithelial to mesenchymal transition (EMT) in ovarian cancer cells

doi: 10.1186/s13048-019-0517-1

Figure Lengend Snippet: Expression of CX3CR1 and CX3CL1 by OvCa cells. OVCAR-3, SW 626, and TOV-112D cells were stained with FITC-conjugated or anti-CX3CR1 and PE-conjugated isotype or anti-CX3CL1 antibodies, and the expressions were quantified by flow cytometry. The gray and white histograms represent isotypes and CX3CR1/CX3CL1 fluorescence intensity, respectively

Article Snippet: After washing with PBS-T, tissue sections were incubated with streptavidin-horseradish peroxidase (HRP, Biolegend) (HIF-1α) or streptavidin-alkaline phosphatase (Jackson ImmunoResearch) (CX3CR1) and developed in diaminobenzidine and/or alkaline phosphatase red chromogen coloring agent.

Techniques: Expressing, Staining, Flow Cytometry, Fluorescence

Journal: Journal of Ovarian Research

Article Title: Hypoxia-inducible factor-1α induces CX3CR1 expression and promotes the epithelial to mesenchymal transition (EMT) in ovarian cancer cells

doi: 10.1186/s13048-019-0517-1

Figure Lengend Snippet: Western blot expression of hypoxia regulatory markers in OvCa cells. Immunoblot detection of OVCAR-3, SW 626, and TOV-112D cells exposed to 3, 6, 9, or 12 h hypoxia respectively. Immunoblotting was accomplished with primary antibodies against HIF-1α, CX3CR1, EMT marker (Snail), and MMP-9. As an internal standard for equal loading, anti-GAPDH antibody was used to probe blots

Article Snippet: After washing with PBS-T, tissue sections were incubated with streptavidin-horseradish peroxidase (HRP, Biolegend) (HIF-1α) or streptavidin-alkaline phosphatase (Jackson ImmunoResearch) (CX3CR1) and developed in diaminobenzidine and/or alkaline phosphatase red chromogen coloring agent.

Techniques: Western Blot, Expressing, Marker

Journal: Journal of Ovarian Research

Article Title: Hypoxia-inducible factor-1α induces CX3CR1 expression and promotes the epithelial to mesenchymal transition (EMT) in ovarian cancer cells

doi: 10.1186/s13048-019-0517-1

Figure Lengend Snippet: mRNA analysis of hypoxia-induced signaling molecules in OvCa cells. Ovarian tumor cells (OVCAR-3, SW 626, and TOV-112D) were exposed to hypoxia for 3, 6, 9, or 12 h. Quantitative RT-PCR results for expressions of CX3CR1, HIF-1α, MMP9, Snail, Twist, N- and P-cadherin are shown. The data were normalized to the levels of the housekeeping gene (18 S), and the analyses were accomplished in triplicate. Data were presented as fold change in expression (± standard error); the asterisks indicate significant differences as determined by Student’s t -test (* P < 0.05; ** P < 0.01)

Article Snippet: After washing with PBS-T, tissue sections were incubated with streptavidin-horseradish peroxidase (HRP, Biolegend) (HIF-1α) or streptavidin-alkaline phosphatase (Jackson ImmunoResearch) (CX3CR1) and developed in diaminobenzidine and/or alkaline phosphatase red chromogen coloring agent.

Techniques: Quantitative RT-PCR, Expressing

Journal: Journal of Ovarian Research

Article Title: Hypoxia-inducible factor-1α induces CX3CR1 expression and promotes the epithelial to mesenchymal transition (EMT) in ovarian cancer cells

doi: 10.1186/s13048-019-0517-1

Figure Lengend Snippet: Migration and invasion induced by the CX3CR1/CX3CL1 interaction in hypoxic OvCa cells. OVCAR-3, SW 626, and TOV-112D cells were cultured in the presence of CoCl 2 (150 μM) (to mimic hypoxia), and with or without CX3CL1 (chemoattractant) and KC7F2 (inhibitor) in the medium. Over 6 days, migration and invasion were assessed. ( a ) Morphology and ( b ) quantitative analysis of surface area, representing the invading cells projecting out of the spheroid into the medium when using CX3CL1. For cells under hypoxia, there was minimal growth, or they remained as aggregates when treated with or without the inhibitor, KC7F2

Article Snippet: After washing with PBS-T, tissue sections were incubated with streptavidin-horseradish peroxidase (HRP, Biolegend) (HIF-1α) or streptavidin-alkaline phosphatase (Jackson ImmunoResearch) (CX3CR1) and developed in diaminobenzidine and/or alkaline phosphatase red chromogen coloring agent.

Techniques: Migration, Cell Culture

Journal: Biomolecules

Article Title: 1-(Arylsulfonyl-isoindol-2-yl)piperazines as 5-HT 6 R Antagonists: Mechanochemical Synthesis, In Vitro Pharmacological Properties and Glioprotective Activity

doi: 10.3390/biom13010012

Figure Lengend Snippet: ( A ): Functional dose-response curve of inhibition of cAMP production at 5-HT 6 R for selected compounds 3e , 3f , and 3g in 1321N1 cells. Data were obtained from three independent experiments run in triplicate. ( B ): Impact of compounds 3e , 3f , and 3g and SB-258585 on basal cAMP production in NG108-15 cells transiently expressing 5-HT 6 R. For each compound, six independent transfection experiments were performed, and data were measured in triplicate. Data are given as means ± SEM of the values.

Article Snippet: The ability of compounds 3e , 3f , and 3g to inhibit 5-CT-induced production of cAMP was assessed using 1321N1 cells expressing the human 5-HT 6 R (PerkinElmer) using previously described procedures [ , ].

Techniques: Functional Assay, Inhibition, Expressing, Transfection

Journal: ACS chemical neuroscience

Article Title: Discovery of Novel Proline-Based Neuropeptide FF Receptor Antagonists

doi: 10.1021/acschemneuro.7b00219

Figure Lengend Snippet: NPFF EC50 in functional calcium mobilization assays. (A) NPFF response in stable NPFF1-RD-HGA16 cells (●) and parental RD-HGA16 CHO cells (○). (B) NPFF response in stable NPFF2-RD-HGA16 cells (■) and parental RD-HGA16 CHO cells (○). Representative data from one experiment is shown and each data point is the mean ± SD of duplicate determinations.

Article Snippet: Stable human NPFFR1-CHO (ES-491-C) and NPFFR2-CHO (ES-490-C) cell lines were purchased from PerkinElmer and used with the Lance Ultra kit (TRF0262) to detect cAMP accumulation in low volume 96-well plates.

Techniques: Functional Assay

Journal: ACS chemical neuroscience

Article Title: Discovery of Novel Proline-Based Neuropeptide FF Receptor Antagonists

doi: 10.1021/acschemneuro.7b00219

Figure Lengend Snippet: Antagonist activity of compound 16 in NPFF1 (A) and NPFF2 (B) calcium mobilization functional Ke assays. (A) Concentration-response curves of NPFF alone (○) and NPFF + 5 μM final 16 (□) in stable NPFF1-RD-HGA16 cells. (B) Concentration-response curves of NPFF alone (○) and NPFF + 10 μM final 16 (□) in stable NPFF2-RD-HGA16 cells. The right shift of the NPFF curve in the presence of test compound was used to calculate Ke values as described in the Methods. Representative data from one experiment are shown and each data point is mean ± SD of duplicate determinations.

Article Snippet: Stable human NPFFR1-CHO (ES-491-C) and NPFFR2-CHO (ES-490-C) cell lines were purchased from PerkinElmer and used with the Lance Ultra kit (TRF0262) to detect cAMP accumulation in low volume 96-well plates.

Techniques: Activity Assay, Functional Assay, Concentration Assay

Journal: ACS chemical neuroscience

Article Title: Discovery of Novel Proline-Based Neuropeptide FF Receptor Antagonists

doi: 10.1021/acschemneuro.7b00219

Figure Lengend Snippet: Antagonist activity of compounds 16 and 33 in NPFF1 (A) and FF2 (B) cAMP functional Ke assays. (A) Concentration-response curves of NPFF alone (○), NPFF + 4 μM final 16 (□), and NPFF + 2 μM final 33 (◊) in stable NPFF1-CHO cells. (B) Concentration-response curves of NPFF alone (○), NPFF + 10 μM final 16 (□), and NPFF + 10 μM final 33 (◊) in stable NPFF2-CHO cells. The right shift of the NPFF curve in the presence of test compound was used to calculcate Ke values as described in the Methods. Each data point is mean ± SEM of at least N=3 conducted in duplicate.

Article Snippet: Stable human NPFFR1-CHO (ES-491-C) and NPFFR2-CHO (ES-490-C) cell lines were purchased from PerkinElmer and used with the Lance Ultra kit (TRF0262) to detect cAMP accumulation in low volume 96-well plates.

Techniques: Activity Assay, Functional Assay, Concentration Assay